Produced:Article Title: Orexin/hypocretin receptor 2 signaling in MCH neurons regulates REM sleep and insulin sensitivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MCH antibody produced in rabbit Sigma-Aldrich Cat# M8440; RRID:AB_260690 dsRed Polyclonal Antibody Takara Bio Cat# 632496; RRID:AB_10013483 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21244; RRID:AB_2535812 Alexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG (H + L) (min X Hu,Ms,Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 111-545-144; RRID:AB_2338052 Chemicals, peptides, and recombinant proteins Buprenorphine Bayer PZN 01498870 Meloxicam Boerhinger Ingelheim PZN 07578423 Tramal Gr€unenthal Gmbh Cat#95005446 Isofluran CP-pharma Cat#1214 Isofluran AbbVie Cat#B506 20% glucose B. Braun N/A Human Insulin Eli Lilly Cat#HP8804 EZ PREP buffer Sigma Cat#D8938 DAPI Thermo Fisher Scientific Cat#62248 Picrotoxin Sigma-Aldrich Cat#P1675 DL-2-amino-5-phosphonopentanoic acid Biotrend Cat#BN0086 6-cyano-7-nitroquinoxaline-2,3-dione Sigma-Aldrich Cat#C127 Amphotericin B Sigma-Aldrich Cat#A4888 Biocytin Sigma-Aldrich Cat#B4261 DMSO Sigma-Aldrich Cat#D8418 Orexin B Sigma-Aldrich Cat#O6262 Roti-Histofix Carl Roth Cat#P0873 Entellan Sigma-Aldrich Cat#107960 Vectashield Antifade Mounting Medium Vector Laboratories Cat#H-1200 VectaMount mounting media Vector Laboratories Cat#H-5000 Haematoxylin Vector Laboratories Cat#H-3404 RNAscope HybEz hybridization system ACD bio/Bio-Techne Cat#321462 RNAscope Protease Plus ACD bio/Bio-Techne Cat#322331 RNAscope Target Retrieval ACD bio/Bio-Techne Cat#322000 Opal 520 Fluorophore Akoya Bioscience Cat#FP1487001KT Opal 570 Fluorophore Akoya Bioscience Cat#FP1488001KT Opal 690 Fluorophore Akoya Bioscience Cat#FP1497001KT Critical commercial assays Super-Bond Universal Kit SUN MEDICAL N/A .. Chromium Single Cell 30 Reagent Kits v3 10x Genomics Cat#1000092 and 1000074 RNAscope Multiplex Fluorescent Detection kit v2 ACD bio/Bio-Techne Cat#323100 RNAscope Hcrtr1 probe ACD bio/Bio-Techne Cat#466631-C2 RNAscope Hcrtr2 probe ACD bio/Bio-Techne Cat#581631-C3 (Continued on next page) Cell Reports 44, 115277, February 25, 2025 11
Recombinant:Article Title: Orexin/hypocretin receptor 2 signaling in MCH neurons regulates REM sleep and insulin sensitivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MCH antibody produced in rabbit Sigma-Aldrich Cat# M8440; RRID:AB_260690 dsRed Polyclonal Antibody Takara Bio Cat# 632496; RRID:AB_10013483 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21244; RRID:AB_2535812 Alexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG (H + L) (min X Hu,Ms,Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 111-545-144; RRID:AB_2338052 Chemicals, peptides, and recombinant proteins Buprenorphine Bayer PZN 01498870 Meloxicam Boerhinger Ingelheim PZN 07578423 Tramal Gr€unenthal Gmbh Cat#95005446 Isofluran CP-pharma Cat#1214 Isofluran AbbVie Cat#B506 20% glucose B. Braun N/A Human Insulin Eli Lilly Cat#HP8804 EZ PREP buffer Sigma Cat#D8938 DAPI Thermo Fisher Scientific Cat#62248 Picrotoxin Sigma-Aldrich Cat#P1675 DL-2-amino-5-phosphonopentanoic acid Biotrend Cat#BN0086 6-cyano-7-nitroquinoxaline-2,3-dione Sigma-Aldrich Cat#C127 Amphotericin B Sigma-Aldrich Cat#A4888 Biocytin Sigma-Aldrich Cat#B4261 DMSO Sigma-Aldrich Cat#D8418 Orexin B Sigma-Aldrich Cat#O6262 Roti-Histofix Carl Roth Cat#P0873 Entellan Sigma-Aldrich Cat#107960 Vectashield Antifade Mounting Medium Vector Laboratories Cat#H-1200 VectaMount mounting media Vector Laboratories Cat#H-5000 Haematoxylin Vector Laboratories Cat#H-3404 RNAscope HybEz hybridization system ACD bio/Bio-Techne Cat#321462 RNAscope Protease Plus ACD bio/Bio-Techne Cat#322331 RNAscope Target Retrieval ACD bio/Bio-Techne Cat#322000 Opal 520 Fluorophore Akoya Bioscience Cat#FP1487001KT Opal 570 Fluorophore Akoya Bioscience Cat#FP1488001KT Opal 690 Fluorophore Akoya Bioscience Cat#FP1497001KT Critical commercial assays Super-Bond Universal Kit SUN MEDICAL N/A .. Chromium Single Cell 30 Reagent Kits v3 10x Genomics Cat#1000092 and 1000074 RNAscope Multiplex Fluorescent Detection kit v2 ACD bio/Bio-Techne Cat#323100 RNAscope Hcrtr1 probe ACD bio/Bio-Techne Cat#466631-C2 RNAscope Hcrtr2 probe ACD bio/Bio-Techne Cat#581631-C3 (Continued on next page) Cell Reports 44, 115277, February 25, 2025 11
Article Title: Disrupted RNA editing in beta cells mimics early-stage type 1 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig anti Insulin Agilent Cat# IR002; RRID:AB_2800361 Goat anti GFP Abcam Cat# Ab6673;RRID:AB_305643 Rabbit anti Iba1 Abcam Cat# Ab178846; RRID:AB_2636859 Rat anti CD3 Abcam Cat# Ab11089; RRID:AB_2889189 Rat anti CD8 Thermo Fisher Cat# 14-0808-80; RRID:AB_2572860 Rat anti CD19 Thermo Fisher Cat# 14-0194-82; RRID:AB_2637171 Rabbit anti CD45 Abcam Cat# Ab10558; RRID:AB_442810 Rabbit anti MafA Bethyl Cat# A700-067; RRID:AB_2891864 Mouse anti Proinsulin Abcam Cat# ab243141; RRID: AB_3076248 Rabbit anti PC1/PC3 Abcam Cat# Ab220363; RRID: AB_3076249 Mouse anti glucagon Abcam Cat #AB10988; RRID:AB_297642 Rabbit anti mCherry Abcam Cat# AB167453RRID:AB_2571870 Rabbit anti ADAR1 Invitrogen Cat # PA5-21369RRID:AB_11154508 Rabbit anti somatostatin Abcam Cat# 108456; RRID:AB_11158517 Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 705-545-147, RRID:AB_2336933 Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu, Ms,Rat,Shp Sr Prot) Jackson ImmunoResearch Labs Cat# 711-165-152, RRID:AB_2307443 Alexa Fluor 647-AffiniPure Donkey Anti-Mouse IgM, m Chain Specific (min X Hu,Bov,Hrs,Rat Sr Prot) antibody Jackson ImmunoResearch Labs Cat# 715-605-140, RRID:AB_2340861 Biological samples Mouse Pancreas This paper N/A Mouse Islets This paper N/A Human Islets Table S1 N/A Chemicals, peptides, and recombinant proteins Tamoxifen Sigma-Aldrich Cat# T5648-5G PerfeCTa SYBR Green FastMix Quanta Biosciences Cat# 95073-012-2 SYBR PCR Master mix Bio-Rad Cat# 1725124 TRIzol Reagent Thermo Fisher Cat# 15596026 Ruxolitinib Abcam Cat# AB-ab141356-25-B GKA Pfizer Cat# HY-108328 Diazoxide Sigma-Aldrich Cat# D9035-250MG Nifedipine Sigma-Aldrich Cat# N7634-1G IFNa BioLegend Cat# BLG-752804 ExtrAvidin-Peroxidase Sigma-Aldrich Cat# E2886 DAB Quanto Thermo Scientific Cat# TA-125-HDX Hematoxylin Sigma-Aldrich N/A Zombie Violet Dye Biolegend Cat# 77477 Perm/Wash Perm/Wash Buffer BD Biosciences Cat# 54723 Cytofix/Cytoperm Fixation and Permeabilization Solution BD Biosciences Cat# 554722 Droplet Generation Oil for Probes Bio-Rad Cat# D9161172A ddPCR Supermix for Probes Bio-Rad Cat# 1863024 (Continued on next page) Cell Metabolism 36, 1–14.e1–e6, January 2, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Direct-Zol RNA-MicroPrep w/ Zymo-Spin IC Columns Zymo Cat# R2062 qScript cDNA synthesis kit Quanta Biosciences Cat# 95047-500 SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian Takara Bio Cat# 634485 The Rat Insulin ELISA (Ultra Sensitive) Crystal Chem Cat#90060 Deposited data Source data for all graphs Data S1 N/A Experimental models: Organisms/strains Mouse: Adarf/f Hartner et al.8 NA Mouse: MIP-CreER Tamarina et al.62 NA Mouse: ROSA26-LSL-EYFP Jackson Laboratory B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J Mouse: Ifih1 knock-out Jackson Laboratory B6.Cg-Ifih1tm1.1Cln/J Software and algorithms Prism (version 8.0.2) GraphPad https://www.graphpad.com/scientific- software/prism/ ImageJ (version 1.8.0) NIH http://imagej.nih.gov/ij GSEA https://www.gsea-msigdb.org/gsea/ index.jsp FastQC (version 0.11.8) Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/fastqc/ UMI-tools (versions 1.1.1 & 1.1.2) https://umi-tools.readthedocs.io/en/latest/ seqtk trimfq https://github.com/lh3/seqtk STAR (version 2.7.3a) https://github.com/alexdobin/STAR htseq-count script (version 2.0.1) https://htseq.readthedocs.io/en/master/ htseqcount.html DeSeq2 (version 1.28.1) https://github.com/mikelove/DESeq2 Other Collagenase P Sigma-Aldrich Cat# 11213873001 RPMI 1640 Gibco Cat# 31870025 RPMI 1640 no glucose Sartorius Cat# 01-101-1A Histopaque-1119 (Ficoll 1.119g/mL) Sigma-Aldrich Cat# 111910-100ML Histopaque- 1077(Ficoll 1.079 g/mL) Sigma-Aldrich Cat# 10771-100ML Poly(I:C) HMW InvivoGen Cat# tlrl-pic
RNAscope:Article Title: Orexin/hypocretin receptor 2 signaling in MCH neurons regulates REM sleep and insulin sensitivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MCH antibody produced in rabbit Sigma-Aldrich Cat# M8440; RRID:AB_260690 dsRed Polyclonal Antibody Takara Bio Cat# 632496; RRID:AB_10013483 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21244; RRID:AB_2535812 Alexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG (H + L) (min X Hu,Ms,Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 111-545-144; RRID:AB_2338052 Chemicals, peptides, and recombinant proteins Buprenorphine Bayer PZN 01498870 Meloxicam Boerhinger Ingelheim PZN 07578423 Tramal Gr€unenthal Gmbh Cat#95005446 Isofluran CP-pharma Cat#1214 Isofluran AbbVie Cat#B506 20% glucose B. Braun N/A Human Insulin Eli Lilly Cat#HP8804 EZ PREP buffer Sigma Cat#D8938 DAPI Thermo Fisher Scientific Cat#62248 Picrotoxin Sigma-Aldrich Cat#P1675 DL-2-amino-5-phosphonopentanoic acid Biotrend Cat#BN0086 6-cyano-7-nitroquinoxaline-2,3-dione Sigma-Aldrich Cat#C127 Amphotericin B Sigma-Aldrich Cat#A4888 Biocytin Sigma-Aldrich Cat#B4261 DMSO Sigma-Aldrich Cat#D8418 Orexin B Sigma-Aldrich Cat#O6262 Roti-Histofix Carl Roth Cat#P0873 Entellan Sigma-Aldrich Cat#107960 Vectashield Antifade Mounting Medium Vector Laboratories Cat#H-1200 VectaMount mounting media Vector Laboratories Cat#H-5000 Haematoxylin Vector Laboratories Cat#H-3404 RNAscope HybEz hybridization system ACD bio/Bio-Techne Cat#321462 RNAscope Protease Plus ACD bio/Bio-Techne Cat#322331 RNAscope Target Retrieval ACD bio/Bio-Techne Cat#322000 Opal 520 Fluorophore Akoya Bioscience Cat#FP1487001KT Opal 570 Fluorophore Akoya Bioscience Cat#FP1488001KT Opal 690 Fluorophore Akoya Bioscience Cat#FP1497001KT Critical commercial assays Super-Bond Universal Kit SUN MEDICAL N/A .. Chromium Single Cell 30 Reagent Kits v3 10x Genomics Cat#1000092 and 1000074 RNAscope Multiplex Fluorescent Detection kit v2 ACD bio/Bio-Techne Cat#323100 RNAscope Hcrtr1 probe ACD bio/Bio-Techne Cat#466631-C2 RNAscope Hcrtr2 probe ACD bio/Bio-Techne Cat#581631-C3 (Continued on next page) Cell Reports 44, 115277, February 25, 2025 11
Hybridization:Article Title: Orexin/hypocretin receptor 2 signaling in MCH neurons regulates REM sleep and insulin sensitivity.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Anti-MCH antibody produced in rabbit Sigma-Aldrich Cat# M8440; RRID:AB_260690 dsRed Polyclonal Antibody Takara Bio Cat# 632496; RRID:AB_10013483 Goat anti-Rabbit IgG (H + L) CrossAdsorbed Secondary Antibody, Alexa FluorTM 647 Invitrogen Cat# A-21244; RRID:AB_2535812 Alexa Fluor 488-AffiniPure Goat Anti-Rabbit IgG (H + L) (min X Hu,Ms,Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 111-545-144; RRID:AB_2338052 Chemicals, peptides, and recombinant proteins Buprenorphine Bayer PZN 01498870 Meloxicam Boerhinger Ingelheim PZN 07578423 Tramal Gr€unenthal Gmbh Cat#95005446 Isofluran CP-pharma Cat#1214 Isofluran AbbVie Cat#B506 20% glucose B. Braun N/A Human Insulin Eli Lilly Cat#HP8804 EZ PREP buffer Sigma Cat#D8938 DAPI Thermo Fisher Scientific Cat#62248 Picrotoxin Sigma-Aldrich Cat#P1675 DL-2-amino-5-phosphonopentanoic acid Biotrend Cat#BN0086 6-cyano-7-nitroquinoxaline-2,3-dione Sigma-Aldrich Cat#C127 Amphotericin B Sigma-Aldrich Cat#A4888 Biocytin Sigma-Aldrich Cat#B4261 DMSO Sigma-Aldrich Cat#D8418 Orexin B Sigma-Aldrich Cat#O6262 Roti-Histofix Carl Roth Cat#P0873 Entellan Sigma-Aldrich Cat#107960 Vectashield Antifade Mounting Medium Vector Laboratories Cat#H-1200 VectaMount mounting media Vector Laboratories Cat#H-5000 Haematoxylin Vector Laboratories Cat#H-3404 RNAscope HybEz hybridization system ACD bio/Bio-Techne Cat#321462 RNAscope Protease Plus ACD bio/Bio-Techne Cat#322331 RNAscope Target Retrieval ACD bio/Bio-Techne Cat#322000 Opal 520 Fluorophore Akoya Bioscience Cat#FP1487001KT Opal 570 Fluorophore Akoya Bioscience Cat#FP1488001KT Opal 690 Fluorophore Akoya Bioscience Cat#FP1497001KT Critical commercial assays Super-Bond Universal Kit SUN MEDICAL N/A .. Chromium Single Cell 30 Reagent Kits v3 10x Genomics Cat#1000092 and 1000074 RNAscope Multiplex Fluorescent Detection kit v2 ACD bio/Bio-Techne Cat#323100 RNAscope Hcrtr1 probe ACD bio/Bio-Techne Cat#466631-C2 RNAscope Hcrtr2 probe ACD bio/Bio-Techne Cat#581631-C3 (Continued on next page) Cell Reports 44, 115277, February 25, 2025 11
SYBR Green Assay:Article Title: Disrupted RNA editing in beta cells mimics early-stage type 1 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig anti Insulin Agilent Cat# IR002; RRID:AB_2800361 Goat anti GFP Abcam Cat# Ab6673;RRID:AB_305643 Rabbit anti Iba1 Abcam Cat# Ab178846; RRID:AB_2636859 Rat anti CD3 Abcam Cat# Ab11089; RRID:AB_2889189 Rat anti CD8 Thermo Fisher Cat# 14-0808-80; RRID:AB_2572860 Rat anti CD19 Thermo Fisher Cat# 14-0194-82; RRID:AB_2637171 Rabbit anti CD45 Abcam Cat# Ab10558; RRID:AB_442810 Rabbit anti MafA Bethyl Cat# A700-067; RRID:AB_2891864 Mouse anti Proinsulin Abcam Cat# ab243141; RRID: AB_3076248 Rabbit anti PC1/PC3 Abcam Cat# Ab220363; RRID: AB_3076249 Mouse anti glucagon Abcam Cat #AB10988; RRID:AB_297642 Rabbit anti mCherry Abcam Cat# AB167453RRID:AB_2571870 Rabbit anti ADAR1 Invitrogen Cat # PA5-21369RRID:AB_11154508 Rabbit anti somatostatin Abcam Cat# 108456; RRID:AB_11158517 Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 705-545-147, RRID:AB_2336933 Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu, Ms,Rat,Shp Sr Prot) Jackson ImmunoResearch Labs Cat# 711-165-152, RRID:AB_2307443 Alexa Fluor 647-AffiniPure Donkey Anti-Mouse IgM, m Chain Specific (min X Hu,Bov,Hrs,Rat Sr Prot) antibody Jackson ImmunoResearch Labs Cat# 715-605-140, RRID:AB_2340861 Biological samples Mouse Pancreas This paper N/A Mouse Islets This paper N/A Human Islets Table S1 N/A Chemicals, peptides, and recombinant proteins Tamoxifen Sigma-Aldrich Cat# T5648-5G PerfeCTa SYBR Green FastMix Quanta Biosciences Cat# 95073-012-2 SYBR PCR Master mix Bio-Rad Cat# 1725124 TRIzol Reagent Thermo Fisher Cat# 15596026 Ruxolitinib Abcam Cat# AB-ab141356-25-B GKA Pfizer Cat# HY-108328 Diazoxide Sigma-Aldrich Cat# D9035-250MG Nifedipine Sigma-Aldrich Cat# N7634-1G IFNa BioLegend Cat# BLG-752804 ExtrAvidin-Peroxidase Sigma-Aldrich Cat# E2886 DAB Quanto Thermo Scientific Cat# TA-125-HDX Hematoxylin Sigma-Aldrich N/A Zombie Violet Dye Biolegend Cat# 77477 Perm/Wash Perm/Wash Buffer BD Biosciences Cat# 54723 Cytofix/Cytoperm Fixation and Permeabilization Solution BD Biosciences Cat# 554722 Droplet Generation Oil for Probes Bio-Rad Cat# D9161172A ddPCR Supermix for Probes Bio-Rad Cat# 1863024 (Continued on next page) Cell Metabolism 36, 1–14.e1–e6, January 2, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Direct-Zol RNA-MicroPrep w/ Zymo-Spin IC Columns Zymo Cat# R2062 qScript cDNA synthesis kit Quanta Biosciences Cat# 95047-500 SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian Takara Bio Cat# 634485 The Rat Insulin ELISA (Ultra Sensitive) Crystal Chem Cat#90060 Deposited data Source data for all graphs Data S1 N/A Experimental models: Organisms/strains Mouse: Adarf/f Hartner et al.8 NA Mouse: MIP-CreER Tamarina et al.62 NA Mouse: ROSA26-LSL-EYFP Jackson Laboratory B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J Mouse: Ifih1 knock-out Jackson Laboratory B6.Cg-Ifih1tm1.1Cln/J Software and algorithms Prism (version 8.0.2) GraphPad https://www.graphpad.com/scientific- software/prism/ ImageJ (version 1.8.0) NIH http://imagej.nih.gov/ij GSEA https://www.gsea-msigdb.org/gsea/ index.jsp FastQC (version 0.11.8) Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/fastqc/ UMI-tools (versions 1.1.1 & 1.1.2) https://umi-tools.readthedocs.io/en/latest/ seqtk trimfq https://github.com/lh3/seqtk STAR (version 2.7.3a) https://github.com/alexdobin/STAR htseq-count script (version 2.0.1) https://htseq.readthedocs.io/en/master/ htseqcount.html DeSeq2 (version 1.28.1) https://github.com/mikelove/DESeq2 Other Collagenase P Sigma-Aldrich Cat# 11213873001 RPMI 1640 Gibco Cat# 31870025 RPMI 1640 no glucose Sartorius Cat# 01-101-1A Histopaque-1119 (Ficoll 1.119g/mL) Sigma-Aldrich Cat# 111910-100ML Histopaque- 1077(Ficoll 1.079 g/mL) Sigma-Aldrich Cat# 10771-100ML Poly(I:C) HMW InvivoGen Cat# tlrl-pic
Polymerase Chain Reaction:Article Title: Disrupted RNA editing in beta cells mimics early-stage type 1 diabetes.
Article Snippet: .. REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Guinea pig anti Insulin Agilent Cat# IR002; RRID:AB_2800361 Goat anti GFP Abcam Cat# Ab6673;RRID:AB_305643 Rabbit anti Iba1 Abcam Cat# Ab178846; RRID:AB_2636859 Rat anti CD3 Abcam Cat# Ab11089; RRID:AB_2889189 Rat anti CD8 Thermo Fisher Cat# 14-0808-80; RRID:AB_2572860 Rat anti CD19 Thermo Fisher Cat# 14-0194-82; RRID:AB_2637171 Rabbit anti CD45 Abcam Cat# Ab10558; RRID:AB_442810 Rabbit anti MafA Bethyl Cat# A700-067; RRID:AB_2891864 Mouse anti Proinsulin Abcam Cat# ab243141; RRID: AB_3076248 Rabbit anti PC1/PC3 Abcam Cat# Ab220363; RRID: AB_3076249 Mouse anti glucagon Abcam Cat #AB10988; RRID:AB_297642 Rabbit anti mCherry Abcam Cat# AB167453RRID:AB_2571870 Rabbit anti ADAR1 Invitrogen Cat # PA5-21369RRID:AB_11154508 Rabbit anti somatostatin Abcam Cat# 108456; RRID:AB_11158517 Alexa Fluor 488 AffiniPure Donkey Anti-Goat IgG (H+L) (min X Ck, GP, Sy Hms, Hrs, Hu, Ms, Rb, Rat Sr Prot) Jackson ImmunoResearch Labs Cat# 705-545-147, RRID:AB_2336933 Cy3-AffiniPure Donkey Anti-Rabbit IgG (H+L) (min X Bov,Ck,Gt,GP,Sy Hms,Hrs,Hu, Ms,Rat,Shp Sr Prot) Jackson ImmunoResearch Labs Cat# 711-165-152, RRID:AB_2307443 Alexa Fluor 647-AffiniPure Donkey Anti-Mouse IgM, m Chain Specific (min X Hu,Bov,Hrs,Rat Sr Prot) antibody Jackson ImmunoResearch Labs Cat# 715-605-140, RRID:AB_2340861 Biological samples Mouse Pancreas This paper N/A Mouse Islets This paper N/A Human Islets Table S1 N/A Chemicals, peptides, and recombinant proteins Tamoxifen Sigma-Aldrich Cat# T5648-5G PerfeCTa SYBR Green FastMix Quanta Biosciences Cat# 95073-012-2 SYBR PCR Master mix Bio-Rad Cat# 1725124 TRIzol Reagent Thermo Fisher Cat# 15596026 Ruxolitinib Abcam Cat# AB-ab141356-25-B GKA Pfizer Cat# HY-108328 Diazoxide Sigma-Aldrich Cat# D9035-250MG Nifedipine Sigma-Aldrich Cat# N7634-1G IFNa BioLegend Cat# BLG-752804 ExtrAvidin-Peroxidase Sigma-Aldrich Cat# E2886 DAB Quanto Thermo Scientific Cat# TA-125-HDX Hematoxylin Sigma-Aldrich N/A Zombie Violet Dye Biolegend Cat# 77477 Perm/Wash Perm/Wash Buffer BD Biosciences Cat# 54723 Cytofix/Cytoperm Fixation and Permeabilization Solution BD Biosciences Cat# 554722 Droplet Generation Oil for Probes Bio-Rad Cat# D9161172A ddPCR Supermix for Probes Bio-Rad Cat# 1863024 (Continued on next page) Cell Metabolism 36, 1–14.e1–e6, January 2, 2024 e1 .. REAGENT or RESOURCE SOURCE IDENTIFIER Critical commercial assays Direct-Zol RNA-MicroPrep w/ Zymo-Spin IC Columns Zymo Cat# R2062 qScript cDNA synthesis kit Quanta Biosciences Cat# 95047-500 SMARTer Stranded Total RNA-Seq Kit v3 – Pico Input Mammalian Takara Bio Cat# 634485 The Rat Insulin ELISA (Ultra Sensitive) Crystal Chem Cat#90060 Deposited data Source data for all graphs Data S1 N/A Experimental models: Organisms/strains Mouse: Adarf/f Hartner et al.8 NA Mouse: MIP-CreER Tamarina et al.62 NA Mouse: ROSA26-LSL-EYFP Jackson Laboratory B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J Mouse: Ifih1 knock-out Jackson Laboratory B6.Cg-Ifih1tm1.1Cln/J Software and algorithms Prism (version 8.0.2) GraphPad https://www.graphpad.com/scientific- software/prism/ ImageJ (version 1.8.0) NIH http://imagej.nih.gov/ij GSEA https://www.gsea-msigdb.org/gsea/ index.jsp FastQC (version 0.11.8) Babraham Institute http://www.bioinformatics.babraham. ac.uk/projects/fastqc/ UMI-tools (versions 1.1.1 & 1.1.2) https://umi-tools.readthedocs.io/en/latest/ seqtk trimfq https://github.com/lh3/seqtk STAR (version 2.7.3a) https://github.com/alexdobin/STAR htseq-count script (version 2.0.1) https://htseq.readthedocs.io/en/master/ htseqcount.html DeSeq2 (version 1.28.1) https://github.com/mikelove/DESeq2 Other Collagenase P Sigma-Aldrich Cat# 11213873001 RPMI 1640 Gibco Cat# 31870025 RPMI 1640 no glucose Sartorius Cat# 01-101-1A Histopaque-1119 (Ficoll 1.119g/mL) Sigma-Aldrich Cat# 111910-100ML Histopaque- 1077(Ficoll 1.079 g/mL) Sigma-Aldrich Cat# 10771-100ML Poly(I:C) HMW InvivoGen Cat# tlrl-pic
High Performance Liquid Chromatography:
Mass Spectrometry:
Knock-Out:
Sequencing:
Software:
other:
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